World number three Pliskova, who had a bye into the final after Britain's Johanna Konta pulled out with injury, has now won three titles this year.
Sixth seed Wozniacki fought hard in the second set but 25-year-old Pliskova's serve was too strong throughout."Hopefully I can go far at Wimbledon," she said.
"I think my service is definitely my biggest weapon and if it's working it causes the other player a lot of problems."Pliskova will go into Wimbledon as one of the favourites for the title, having reached her first Grand Slam final at the US Open last year and the French Open semi-finals at Roland Garros last month.
However, she has never made it past the second round in five appearances at Wimbledon.The last Czech player to win the Eastbourne title was Jana Novotna in 1998, who then went on to triumph at Wimbledon two weeks later.
Wozniacki, who beat Heather Watson in the semi-finals, struggled to cope with the power of Pliskova's serve."I thought I played really well," said the Dane. "But Karolina played better than me definitely with the serves. I had trouble getting them back."